Serial Dilution | Science Primer (2024)

FAQs

How do you answer serial dilution questions? ›

In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=ViVf = 1mL10mL=110 .

Serial dilutions are often performed in steps of 10 or 100. They are described as ratios of the initial and final concentrations. For example, a 1:10 dilution is a mixture of one part of a solution and nine parts fresh solvent. For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent.

Serial Dilution

Therefore, the amount of stock in each ml of the diluted solution is 0.1 ml. This technique used to make a single dilution is repeated sequentially using more and more dilute solutions as the "stock" solution.

A ten-fold dilution reduces the concentration of a solution or a suspension of virus by a factor of ten that is to one-tenth the original concentration. A series of ten-fold dilutions is described as ten-fold serial dilutions.

To perform serial dilution, for example, 1 ml of the starting sample is added to 9 ml of Dilution Blank Tube 1. This is then followed by the same procedure, where 1 ml from Tube 1 is added to 9 ml of Tube 2, 1 ml from Tube 2 is added to 9 ml from Tube 3, and so on until the desired concentration is reached.

What is the dilution factor when 0.2 ml is added to 3.8 ml diluent? 4.0/0.2 = 1:20 dilution.

For example, to make a 1:10 dilution of a 1M NaCl solution, you would mix one "part" of the 1M solution with nine "parts" of solvent (probably water), for a total of ten "parts." Therefore, 1:10 dilution means 1 part + 9 parts of water (or other diluent).

The recommended primer concentration for standard PCR applications is between 0.1μM and 1μM of each primer. Generally, using a low primer concentration is better. It ensures a lower background and cleaner product.

A 1:5 dilution really means - one part, in a total of 5 parts. If 1ml is added to 4mls, you are creating a mixture with 5 total parts (see figure 1 below). This might also be referred to as a 5 fold dilution.

You could make 1/1,000 by adding 1 microliter of sample to 0.999 ml diluent.

What is an example of a 1 10 dilution? ›

Commonly used dilutions are 1:10 or 1:2. Note that this is expressed as the ratio of the previous solution to the final volume of the dilution. For example, to make a 1:10 dilution, you add 1ml of your solution to 9ml of diluent for a final volume of 10ml.

To complete a tenfold dilution, the ratio must be 1:10. The 1 represents the amount of sample added. The 10 represents the total size of the final sample. For example, a sample size of 1 ml is added to 9 ml of diluent to equal a total of 10 ml.

Let's say you want to dilute a stock solution with water to make a working solution that is one tenth as concentrated. This could also be called a ten-fold dilution or a “10X” dilution, because the working solution will be ten times as dilute as the stock. Here the dilution factor is 10.

A 10x dilution is obtained by mixing 1 part of a sample with 9 parts of a diluent so that the new solution is 10 times (10x) less concentrated than the original solution. The 10x dilution can then be diluted by a factor of 10 again by mixing it with 9 more parts of the diluent.

A Serial dilution is a series of dilutions, with the dilution factor staying the same for each step. The concentration factor is the initial volume divided by the final solution volume. The dilution factor is the inverse of the concentration factor.

Serial dilution is a process through which the concentration of an organism, bacteria in this example, is systematically reduced through successive resuspension in fixed volumes of liquid diluent. Usually the volume of the diluent is a multiple of 10 to facilitate logarithmic reduction of the sample organism.

A serial dilution is the stepwise dilution of a substance in a solution. Serial dilutions result in the geometric progression of the concentration in a logarithmic fashion, meaning the concentration decreases by the same quantity in each successive step.

A stepwise dilution of Ligand in Buffer. Typically, a serial 1:1 dilution (dilution factor 2 in DI. Control software) is performed by transferring one volume of ligand solution to an equal volume of buffer, mixing, and repeating this step.