Biosafety (2024)

Table of Contents
BSL-1 BSL-2 BSL-3 BSL-4 FAQs

Biological agents, plant or animal, are classified according to biosafety level risk groups. These classifications presume ordinary circ*mstances in the research laboratory, or growth of agents in small volumes for diagnostic and experimental purposes.

BSL-1

BSL-1 risk group contains biological agents that pose low risk to personnel and the environment. These agents are highly unlikely to cause disease in healthy laboratory workers, animals or plants. The agents require Biosafety Level 1 containment. Examples of BSL-1 organisms are: Agrobacterium radiobacter, Aspergillus niger, Bacillus thuringiensis, Escherichia coli strain K12, Lactobacillus acidophilus, Micrococcus leuteus, Neurospora crassa, Pseudomonas fluorescens, Serratia marcescens.

BSL-2

BSL-2 risk group contains biological agents that pose moderate risk to personnel and the environment. If exposure occurs in a laboratory situation, the risk of spread is limited and it rarely would cause infection that would lead to serious disease. Effective treatment and preventive measures are available in the event that an infection occurs. The agents require Biosafety Level 2 containment. Examples of BSL-2 organisms are: Mycobacterium, Streptococcus pneumonia, Salmonella choleraesuis.

BSL-3

BSL-3 risk group contains biological agents that usually cause serious disease (human, animal or plant) or that can result in serious economic consequences. These agents are usually not spread by casual contact. The agents require Biosafety Level 3 containment.

BSL-4

BSL-4 risk group contains biological agents that usually produce very serious disease (human, animal or plant) that is often untreatable. These agents are usually easily transmitted from one individual to another, from animal to human or vice-versa, either directly or indirectly, or by casual contact. The agents require Biosafety Level 4 containment.

For comments or questions about Ohio Wesleyan Universities Biosafety Plan please contact the Environmental Health and Safety Office at 740-368-3502.

BSL level information is from the Center for Disease Control.

Biosafety (2024)

FAQs

Biosafety? ›

Biosafety is the safe working practices associated with handling of biological materials, particularly infectious agents. It addresses containment principles, technologies and practices that are implemented to prevent the unintentional exposure to pathogens and toxins, or their accidental release.

What do you mean by biosafety? ›

According to the World Health Organisation (WHO), "biosafety is a strategic and integrated approach to analysing and managing relevant risks to human, animal and plant life and health and associated risks for the environment.

What are the 4 types of biosafety? ›

Biological Safety Levels (BSL) are a series of protections relegated to the activities that take place in particular biological labs. They are individual safeguards designed to protect laboratory personnel, as well as the surrounding environment and community.

What is the main goal of biosafety? ›

The basic objective of a biosafety program is the containment of potentially harmful biological agents. The purpose of containment is to reduce or eliminate exposure of laboratory workers, other persons, and the outside environment to potentially hazardous agents.

What is an example of biosafety? ›

Examples of such measures include: biosafety cabinets; personal protective equipment including masks, gloves, safety glasses, and lab coats; hand washing; and.

What is the difference between biosafety and biohazard? ›

Biohazard levels, more commonly referred to as “biological safety levels” or “biosafety levels,” are classifications of safety precautions necessary to be applied in the clinical microbiology laboratory depending on specific pathogens handled when performing laboratory procedures.

What are biosafety rules? ›

Biosafety guidelines are a set of policies, rules, and procedures necessary to observe by personnel working in various facilities handling microbiological agents such as bacteria, viruses, parasites, fungi, prions, and other related agents and microbiological products.

What are the 5 P's of biosafety? ›

The 5 P's. Pathogen, Personnel, Place, PPE and Procedures; these are the five facets that are paramount when performing a risk assessment analysis. Each aspect interacts with the other to determine the level or risk and the controls needed to diminish health hazards.

What are the 5 pillars of biosafety? ›

A biosecurity program rests upon five pillars: inventory process, physical security, a personal reliability program, transport programs, and information security processes. A biosecurity program must have an overall program management that supports the five pillars.

How to determine biosafety level? ›

There are four biosafety levels. Each level has specific controls for containment of microbes and biological agents. The primary risks that determine levels of containment are infectivity, severity of disease, transmissibility, and the nature of the work conducted.

What is the role of biosafety in human life? ›

Biosafety is the prevention of large-scale loss of biological integrity, focusing both on ecology and human health. These prevention mechanisms include the conduction of regular reviews of biosafety in laboratory settings, as well as strict guidelines to follow. Biosafety is used to protect from harmful incidents.

What is the key principle of biosafety? ›

Laboratory biosafety practices are based on the principle of containment of biological agents to prevent exposure to laboratory workers and the outside environment. Primary containment protects the laboratory workers and the immediate laboratory environment from exposure to biological agents.

What are the best practices for biosafety? ›

Hand washing after handling biologicals and potentially hazardous materials, after taking off gloves and before leaving the lab. Avoiding hand-to-face (or mouth) contact. No eating, drinking, smoking, or applying cosmetics in the lab. Disinfecting work surfaces daily and decontaminating after spills.

What are the basic biosafety practices? ›

Basic Laboratory Practices
Biosafety PracticeRoutes of Exposure Blocked
Decontaminate work surfaces before and after use, and immediately after spillsIngestion, skin and mucous membrane contact
Do not eat, drink, store foods, or smoke in the laboratoryIngestion, skin and mucous membrane contact
5 more rows

What are biosafety issues? ›

Biosafety issues refer to the procedures, policies, and principles to be adopted to safeguard the environment and the human population. It refers to the containment principles, strategies, and practices that are adopted to prevent exposure to pathogens and toxins.

What is the principle of biosafety? ›

Laboratory biosafety practices are based on the principle of containment of biological agents to prevent exposure to laboratory workers and the outside environment. Primary containment protects the laboratory workers and the immediate laboratory environment from exposure to biological agents.

What does biosafety level do? ›

Biosafety levels (BSL) are used to identify the protective measures needed in a laboratory setting to protect workers, the environment, and the public. The levels are defined in Biosafety in Biomedical Laboratories (the BMBL).

What are the three classes of biosafety? ›

Biosafety cabinets are divided into three classes: I, II and III. Class I provides protection for the user and surrounding environment, but no protection for the sample being manipulated. Class II provides protection for the user, environment and sample, and is divided into four types: A1, A2, B1 and B2.

What is biosafety risk? ›

Biosafety Risk Assessment, which helps to identify the probability and consequences of infection, is used to ensure that all people potentially exposed to biohazards have an awareness of the potential risk.

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