5 Creative Ways to Teach Pipetting (2024)

When it comes to introducing students to the daily laboratory handling practices, one technique that stands out as a foundational skill is “pipetting”. Pipetting serves as the cornerstone of many scientific experiments as it allows researchers to precisely measure and transfer liquids in a laboratory setting.

From preparing solutions, creating dilutions, transferring reagents to dispensing samples for analysis, pipettes allow us to measure small microliter volumes or larger milliliter quantities with the precision that ensures the reproducibility and accuracy of our experiments.

Educators are often challenged when introducing this lab technique to students for the first time. As this skill is honed over time and practice, there is a catch to it. Only by taking care of small lessons, can we make this topic indulging for students. So, we present 5 such innovative ways to do so!

1. Use Interactive Models and Simulations

When it comes to teaching practical lab handling techniques like pipetting in classes, student engagement to the maximum possible extent is vital for effective learning.

Virtual simulations can help you in replicating real-life lab pipetting scenarios in a safe and controlled environment. These simulated environments possess a range of intrinsic benefits. Students can observe the different parts of micropipettes, and their functions, and learn how to handle them effectively. They can also practice adjusting the volume on different pipette models, master the usage of the plunger’s two stops, and select the appropriate pipette based on the desired volume range. Since this is a virtual simulated world, students don’t have to fear making any mistakes as they can repeat, refine and learn to achieve proficiency which eventually promotes a deeper understanding of the pipetting process.

5 Creative Ways to Teach Pipetting (1)

2. Learning with Games and Activities

Studies have shown that through the utilization of gamification tools, educators can transform the way they deliver points in class, here is about pipetting. (1, 2) Games can serve as effective instructional strategies to teach the intricacies of pipetting techniques.

You can plan to use virtual games where students are given different volumes of chemicals to pipette in a stipulated timeframe. This race will encourage developing precision as well as speed to handle multiple pipettes at a time. It will also reinforce their concept of adjusting the volume on different pipette models. Labster provides such a Pipetting Game where students can learn all of this in a single activity.

3. Infusing Technology into Study Plans

By incorporating various technological resources and interventions, educators can enhance students’ practical skills and conceptual knowledge of pipetting. We suggest some practical ways to do that in your next class.

  • The use of visual aids as in Labster’s Virtual Labs can help in facilitating better comprehension of this technique. As students gain firsthand lab experience, they will get proficient in how to quickly practice unit conversions and apply that to the selection of micropipettes. They will also understand the importance of a well-calibrated pipette.
  • The use of collaborative platforms can also be beneficial as students can engage in group discussions, share resources, and collaborate across the globe. A brilliant platform that answers all such pipetting-related questions in ResearchGate which encourages peer-to-peer learning and provides amazing opportunities for students to exchange tips and tricks of pipetting.

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Discover Labster's Pipetting virtual lab!

4. Inspiring Learners by Connecting to Career Prospects

Inspiring your students by quoting the future prospective career paths related to a topic can work magic while teaching. We recommend that you prioritize motivating your students through examples of scientists, researchers, and prolific biotech R&Ds that are continuously working to bring to the limelight many important biological insights through their continuous efforts in labs where one of the most basic yet important lab skills is pipetting!

5. Connecting the Topic to Real-World Applications

By demonstrating the practical relevance of this topic, educators can enhance students’ proficiency in handling a micropipette. You can talk in-depth about how a micropipette allows researchers to conduct micro-scale experiments with precision and why such experiments are important for the advancement of medical and biotechnology fields.

Labster’s Pipetting Virtual Lab simplifies the science of pipetting by incorporating a real-life scenario that revolves around a fictional company LabsterFood. As students virtually engage in a hands-on experiment to determine the protein content of a corn cob, they learn while being immersed in the simulation. With continued guidance on selecting the appropriate pipette for different volume ranges and valuable tips and tricks, students get to enhance their accuracy in pipetting.

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Final thoughts

Educating our students about the proper usage of lab equipment is of paramount importance. Pipetting being one of them requires time to be perfected. This is why we suggest educators use Labster’s Pipetting Selection Simulation and Pipetting Mastering Simulation to start them early. With newer ways of teaching, we can prepare them better for the goals of the future!

References
  1. Göksün, D. O., & Gürsoy, G. (2019). Comparing success and engagement in gamified learning experiences via Kahoot and Quizizz. Computers & Education, 135, 15-29.
  2. Kalogiannakis, M., Papadakis, S., & Zourmpakis, A. I. (2021). Gamification in science education. A systematic review of the literature. Education Sciences, 11(1), 22.
5 Creative Ways to Teach Pipetting (2024)

FAQs

How to practice pipetting at home? ›

Practice precisely measuring and mixing small volumes using a Pipette Practice Card and colored dyes. Next, learn the art of loading an electrophoresis gel, a critical tool in the molecular biology lab. Instead of the agarose gel you might encounter in the lab, you'll use a sturdy, reusable silicone practice gel.

What are the three rules of pipetting? ›

The following rules apply to all types of pipettes. Never put a pipette in your mouth. Draw the liquid into the pipette using a rubber bulb or pipette pump. Never withdraw a liquid from a near-empty container.

What are the 2 ways of pipetting? ›

Forward and reverse pipetting are two basic pipetting modes that can be used with a mechanical pipette. In forward pipetting, the target volume is aspirated and dispensed, and a separate blowout step is used to completely empty the tip by pressing the plunger to the second stop.

How to test pipetting skills? ›

Pipette Testing Procedure

Draw up a sample of water and dispense it into your weighing vessel. The elegance of this procedure lies in the near-perfect conversion of microliters of distilled water to grams of mass. This conversion involves nothing more than moving the decimal three (3) places.

What are the commandments in pipetting? ›

The commandments instruct the user to discard broken pipettes, ensure pipettes drain fully, control delivery time for accuracy, wipe pipettes before adjusting volume, have low trust in calibration marks, use certified pipettes for accuracy, and deliver viscous liquids like blood slowly from pipettes.

How to avoid bubbles when pipetting? ›

Pipetting slowly, using the reverse pipetting technique reduces the risk of bubble formation when transferring viscous solutions (3). Reverse pipetting is also recommended when pipetting small volumes into 96 well micro test plates for ELISA techniques.

What are the principles of pipetting? ›

The general principle of using a pipette is that the user will set a volume to aspirate (pull in) and dispense (push out). Liquid is pulled into an attached pipette tip, which is uniquely chosen for the volume being handled.

Is it OK to pipette by mouth? ›

Do not ever use your mouth to pull the liquid into a pipet. This is the most common method of becoming poisoned in a chemical laboratory or becoming infected in a clinical laboratory. Mouth pipetting is forbidden in the chemistry department. Do not allow the solution to be drawn up into the bulb.

How to improve pipetting precision? ›

Step 1: The tip is immersed to the correct depth and correctly held vertically. Step 2: Inaccuracy doubles when immersing the tip too deeply. Step 3: Inaccuracy increases three to five times by immersing too deeply while holding the pipette at a 30-40° angle. Use high-quality tips intended for use with the pipette.

What is the first thing you should do when using a pipet? ›

The first step when using a pipette is setting the pipette to your desired volume. To do this, locate the small window on your pipette that has three numbers in it. If your pipette has a lock/unlock switch, switch it to the unlock position.

What is forward pipetting technique? ›

In forward pipetting, an exactly set volume of liquid is aspired to the tip and then it is delivered to a new vessel. This is achieved by creating vacuum by means of the vertical travel of a metal or ceramic piston within an airtight sleeve.

Is pipetting a lab skill? ›

The ability to use micropipettes is a valuable skill for employment in any biotech lab. In order to develop a skill with accuracy, students need to practice.

What is the procedure of handling pipette? ›

Place either your index finger or thumb over the top of plunger and press to the first stopping point. Hold the plunger in this position. Place your pipette tip into your sample and let up on the plunger to allow the sample to rise into the tip. Also, make sure your pipette is vertical or within a 20 degree angle.

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